Journal: Journal of visualized experiments : JoVE
Article Title: Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
doi: 10.3791/60716
Figure Lengend Snippet: Table of Materials
Article Snippet: 14–635-5D Dumont #5 Forceps Fine Science Tools 11252–20 Egg collection cage FlyStuff 59–100 FemtoJet 5247 Eppendorf discontinued FemtoJet 4i (Cat No. 5252000021) ImageJ NIH Image processing software Micromanipulator Sutter MP-225 Micropipette beveler Sutter BV-10-B Needle puller Narishige PC-100 Nutri-Fly Grape Agar Powder Premix Packets FlyStuff 47–102 Nylon Net Filter Millipore Paraformaldehyde 16% Solution, EM grade Electron Microscopy Sciences 15710 Any EM grades PBS Roche 11666789001 Sold on sigmaaldrich, boxed 10x solution Photo-Flo 200 Kodak 146 4510 Upright fluorescence microscope Nikon N/A Eclipse Ci with a LED light source Vinyl Electrical Tape Scotch 6143 VWR Cell Strainers VWR 10199–659 Yeast FlyStuff 62–103 Active dry yeast (RED STAR) Open in a separate window NOTE: Alternatively, cell strainers with pores of 100 μm ( ) can be used for the filtration step of embryo collection.
Techniques: Microscopy, Software, Electron Microscopy, Fluorescence